AB血清對人體外終末期紅系分化進程的影響

摘要

目的：探究體外誘導造血干細胞向紅系分化過程中人AB血清對終末期紅系分化進程的影響。

方法：從臍帶血中用免疫磁珠法分離提取CD34+細胞，體外誘導其向紅系分化。根據培養體系中是否添加人AB血清，設置實驗組為添加人AB血清組，對照組為不添加人AB血清組。通過檢測細胞表面紅系分化標志分子糖化血紅蛋白A(glycophorin A,GPA)、人紅細胞陰離子交換蛋白Band3及整合素α4(α4-integrin)的表達反映終末紅系分化進程,用流式細胞術檢測紅系細胞終末分化脫核情況。結果:與對照組相比,實驗組GPA陽性細胞百分率較高,Band3表達較高,而α4-integrin表達較低(P<0.05)。實驗組細胞脫核百分率明顯高于對照組(P<0.05)。結論:在體外紅系發育培養體系中,人AB血清對終末期紅系分化進程有一定的促分化、促脫核作用。

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Dec 28;41(12):1245-1251. doi: 10.11817/j.issn.1672-7347.2016.12.001.

[Effect of AB serum on human terminal erythroid differentiation ex vivo].

[Article in Chinese; Abstract available in Chinese from the publisher]

Liao J1,et al.

Abstract in English, Chinese

OBJECTIVE:

To demonstrate the effect of AB serum on terminal erythroid differentiation ex vivo.? Methods: After separation of CD34+ cells from cord blood, the cells were cultured and divided into a control group and an experimental group. The effects of AB serum were examined by the expressions of different markers (GPA, Band3 and α4-integrin) for erythroblast differentiation and enucleation by flow cytometry. Results: The CD34+ cells were successfully differentiated to enucleated red blood cells. There were evident differences among the expressions of GPA, Band3 and α4-integrin between the 2 groups. The percentage of GPA positive cells in the experimental group was bigger than that in the control group in every time point. The expression of Band3 in the experimental group was higher than that in the control group. The expression of α4-integrin in the experimental group was lower than that in the control group. In addition, the enucleation rate in the experimental group was higher than that in the control group.? Conclusion: AB serum can promote the cell differentiation and enucleation during terminal erythroid differentiation in vitro.

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GemCell™人AB血清來自于美國FDA認證的AB血型健康男性供體。供體AB血漿加抗凝劑經去纖維蛋白處理得到AB血清，一致性較高。所有供體的病毒標志物檢測都是陰性。

人AB血清作為牛血清（FBS、FCS）的替代物，不含外源物，適合組織工程、移植和細胞治療領域的間充質干細胞擴增，及其他免疫系統相關的細胞培養和反應。

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紅榮微再（上海）生物工程技術有限公司以“傳遞科學價值，服務科學研究”為宗旨，主營：干細胞、醫療、細胞治療、器官再生四大板塊的產品。