Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系統，客戶既可以對某個特殊病人來源的iPS實現轉化，也可以利用cellartis供應的iPS進行標準化操作。使用本產品的過程起始于誘導人iPS轉化成為確定性內胚層（Definitive Endoderm (DE)）細胞，然后誘導轉化DE至肝細胞。本產品是All-In-One型產品，實驗所需的培養基、基質（coating）等包括在內。應用本產品轉化約3 × 10⁶ hiPS cells,可以獲得約5 × 10⁶ 肝細胞（大約50 cm²面積的單層貼壁細胞）。

利用Cellartis iPS Cell to Hepatocyte Differentiation System轉化得到的肝細胞除了清晰表現出肝細胞的*特征外（如肝細胞特異性標志物、CYP系列酶等），還保留了原始細胞供體者的遺傳基因背景。另外，相比較從組織切除獲得的原代肝臟細胞（通常需要低溫保存），通過本產品系統轉化獲得的肝細胞在更長的時間跨度上保持著生理功能的穩定性。這一點對于毒理學評估和病毒感染評估具有顯著的意義。為長時間維持培養hiPS轉化而來的肝細胞，我們推薦Cellartis Hepatocyte Maintenance Medium (Cat. No. Y30051)。

關于Cellartis 的iPS Cell to Hepatocyte Differentiation技術的專項說明，咨詢。

紅榮微再（上海）生物工程技術有限公司    152 1681 4001    Phone 

■ Cellartis iPS Cell to Hepatocyte Differentiation System 產品特點

· Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS

   cell lines, so no need to optimize for your lines.

· Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS

   cell-derived hepatocytes with diverse genetic backgrounds.

· Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver

   disease models.

· Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.

· Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.

■ 產品成分

· Cellartis DEF-CS 100 Culture System (Y30020, not sold separay)

· Cellartis Definitive Endoderm Differentiation Kit (Y30030, not sold separay)

· Cellartis Hepatocyte Differentiation Kit (Y30050)

歡迎您致電 華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司 ：152 1681 4001。華雅再生醫學-客服: 316 808 6348

Cellartis iPS Cell to Hepatocyte Differentiation System 產品訂購清單

Y30050     Cellartis® Hepatocyte Differentiation Kit                          

Y30051     Cellartis® Hepatocyte Maintenance Medium                           

Y30035     Cellartis® Definitive Endoderm Differentiation Kit with DEF-CS™                            

組份-1      Cellartis DEF-CS 100 Culture System (Cat. No. Y30020                    

組份-2      Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030

"Cat. No. Y30020 Package Contents

Package 1:

· 100 ml DEF-CS 100 Basal Medium

· 800 μl DEF-CS COAT-1

Package 2:

· 300 μl DEF-CS GF-1

· 100 μl DEF-CS GF-2

· 40 μl DEF-CS GF-3

Cat. No. Y30030 Package Contents

? 10 ml Definitive Endoderm Differentiation Coating

? 18 ml Definitive Endoderm Differentiation Day 0

? 18 ml Definitive Endoderm Differentiation Day 1

? 18 ml Definitive Endoderm Differentiation Day 2

? 25 ml Definitive Endoderm Differentiation Day 3

? 47 ml Definitive Endoderm Differentiation Day 4

? 47 ml Definitive Endoderm Differentiation Day 6

歡迎您致電 華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司 ：152 1681 4001。華雅再生醫學-客服: 316 808 6348