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英文名稱：Cellartis GS1‐R Rat pluripotent stem cell culture medium

中文名稱：Cellartis GS1‐R大鼠多能干細胞培養基

用途：大鼠ES和EG細胞的分離培養和長期維持生殖潛力

保存：基礎培養基-20ºC；添加劑-80ºC

GS1‐R是Cellartis推出的一款專門用于大鼠（Rats）胚胎干細胞（ES）或胚胎生殖細胞（EG）的培養基。該產品中的成分可以壓抑細胞分化信號，從而很好地把培養的細胞保持在未分化的基態（ground state）,與此同時細胞的活性和增殖不受影響。在本產品中培養增殖的大鼠細胞可以用于向生殖細胞發育相關的研究潛力，可以用來構造轉基因大鼠，比如通過傳統的homologous recombination方法或是 nuclease-based gene editing方法。GS1‐R是成分確定的培養基，無血清添加，需要飼養層。

GS1‐R is a compley defined, serum‐free cell culture medium that is used for the derivation, maintenance, and propagation of rat embryonic stem (ES) and embryonic germ (EG) cells. The medium contains selective small molecule inhibitors that block differentiation-inducing signals, maintaining cells in the ‘ground state’ of self-renewal and promoting cell survival and growth (1–3). Rat ES cells cultured in GS1‐R medium are germ-line competent and can be used to generate transgenic knock‐out rats using either classic homologous recombination or nuclease-based gene editing techniques.

產品特點

成分*確定的配方；無血清

利用選擇性小分子抑制劑阻斷來自GSK3β和ERK / MEK的分化信號

在該培養基中培養可以有效地擴增大鼠ES細胞并進行遺傳操作

Compley defined formulation; serum-free

Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK

Culture in this medium allows rat ES cells to be efficiently derived, propagated, and genetically manipulated

產品應用(ES：胚胎干細胞；EG：胚胎生殖細胞）

大鼠ES和EG細胞的分離培養和長期維持生殖潛力

Derivation and long‐term maintenance of germline-competent rat ES and EG cells

組份：培養基+添加劑

100 ml medium

100 µl 2i inhibitor supplement

自備試劑：飼養層細胞或預鋪培養容器+LIF

Rat ES and EG cells will require co-culture support from feeder cells; Stem Cell Sciences (UK) Ltd. recommends inactivated mouse embryonic fibroblasts seeded at 2 - 4 x 104 cells/cm2 on pre-gelatinised culture vessels.Further supplementation with an activating STAT3 signalling cytokine such as LIF is optional5.

大鼠ES和EG細胞需要來自飼養細胞的共培養支持; Stem Cell Sciences（UK）Ltd.推薦滅活的小鼠胚胎成纖維細胞接種2-4×10 ⁴個細胞/ cm ²在pre-gelatinised的培養容器上。進一步補充活化STAT3信號傳導因子如LIF。

保存：Upon receipt, store the media at -20℃ and the 2i supplement at -80℃ until ready to use.When stored under these conditions, the products are stable for 12 months from the date of manufacture (see label).Once thawed and combined, store at 4℃ and use within 2 weeks.This product is light sensitive, and should be protected from light.

參考文獻：

1. Li P, et al. (2008) Germline competent embryonic stem cells derived from rat blastocysts. Cell. 135(7): 1299-1310.

2.Buehr M, et al. (2008) Capture of authentic embryonic stem cells from rat blastocysts. Cell. 135(7): 1287-1298.

3.Leitch H, et al. (2013) Naive pluripotency is associated with global DNA hypo-methylation. Nat Struct Mol Biol. 20: 311–316.

4.Tong C, et al. (2011) Generating gene knockout rats by homologous recom-bination in embryonic stem cells. Nat Protocols. 6: 827-844.

5.Blair K, et al. (2012) Culture parameters for stable expansion, genetic modi-fication and germline transmission of rat pluripotent stem cells. Biol Open.1(1): 58-65.

6.Tong C, et al. (2012) Rapid and cost-effective gene targeting in rat embryonic stem cells by TALENs. J Genet Genomics. 39(6): 275-280.

Cellartis 小鼠/大鼠胚胎干細胞培養基系列：成分確定、無血清培養基

GS2-M® 小鼠胚胎干細胞和多能誘導干細胞培養基，無飼養層，不依賴LIF

iSTEM™ 小鼠胚胎干細胞培養基，難獲得ES細胞小鼠

GS1-R® 大鼠多能干細胞培養基，需要飼養層，胚胎干細胞和胚胎生殖細胞（EG）

華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司主營細胞治療和精準醫療等產品，2018年與TAKARA繼續合作，授權代理（上海）其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構建試劑。Cellartis公司位于瑞典，擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導技術和ES細胞，ips細胞，分化細胞等干細胞相關產品。

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