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人原代肝臟細胞培養基

人原代肝臟細胞（Human primary hepatocytes, hphep) 是肝臟疾病毒理學研究和相關藥物研發的有力工具。對肝臟疾病模型研究和肝毒性檢測而言，一個穩健的hphep培養體系至關重要。然而，當前可用的培養體系培養的肝臟細胞在一周內就喪失了功能性和活性，嚴重限制了原代肝臟細胞的應用。而且，這些培養基維持培養中還需要每天換液。

Cellartis Power Primary HEP Medium是專門制定的、用于長期維持培養的人原代肝臟細胞培養基。凍存復蘇后接種的hphep，在方便操作的2D培養中，可以存活并維持功能特性4周。此外，這款培養基進一步簡化了肝細胞培養，提供一個周末免換液的培養日程。

Cellartis Power Primary HEP Medium

Human primary hepatocytes (hphep) are an invaluable tool for academic and pharmaceutical labs studying liver disease pathology or as part of the drug development pipeline. To assess disease models and hepatocyte toxicity, robust and predictive hphep cell cultures are required. However, currently available culture media severely limits the utility of primary hepatocytes because hepatocytes lose their function and viability within a week. Additionally, daily feedings are required to maintain the hphep cultures.

產品特點

☆延長培養時間—原代肝臟細胞可以存活并維持功能特性長達4周，經過CYP 活性、 albumin分泌和CYP誘導能力檢測驗證。

☆形式靈活—冷凍運輸，可以被分裝和再次凍存。

☆操作簡便—周末免換液的培養日程, 使用隔天換液 (周一，周三，周五) 而不是每天換液 ☆無需準備復雜的overlays或sandwich培養。

產品應用

肝臟疾病建模Liver disease modeling

肝毒性評估Hepatotoxicity assessment

慢性中毒 Chronic toxicity

組份：250mL培養基

250 ml Cellartis Power Primary HEP Medium (Cat. No. Y20020)

自備試劑：肝細胞+膠原蛋白或膠原蛋白預包被培養板+PBS

•General cell culture equipment

•Human cryoplateable primary hepatocytes with the providers recommended thawing and plating medium

•Collagen I from rat tail (e.g., Corning Cat. No. 354236) or pre-coated Collagen I plates (e.g., Corning BioCoat™ Cat. No. 354407 or 354408)

•PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)

If applicable:

• Penicillin-Streptomycin (PEST) for your thawing and plating medium (see section V.A.1)

培養性能

使用Cellartis Power Primary HEP Medium分別培養來源于三個不同品牌的、復蘇接種的人原代肝臟細胞。

Cryoplateable human primary hepatocytes from three different vendors (1, 2, and 3) cultured in Cellartis Power Primary HEP Medium. Cells were cultured as described and images were taken 1 (Panel A), 7 (Panel B), 14 (Panel C), 21 (Panel D) and 28 (Panel E) days after thawing. For all images, the scale bar is 100 μm

新型的Cellartis Power Primary HEP Medium是專門研發的、用于長期維持培養的人原代肝臟細胞培養基。目前市場上銷售的原代肝臟細胞培養都配套有優化的培養體系，主要有2D和3D培養兩種形式。2D貼壁培養，操作簡便，但是培養的肝細胞在2-7天失去表型，而且要求每天換液。3D懸浮培養雖然可以延長肝臟細胞功能特性維持的時間，但是在操作上比較復雜，需要制備overlay進行sandwich 培養 (Liu et al. 1999)或借助生物反應器Bioreactors (Hoffman et al. 2012)等，成本也較高。Cellartis Power Primary HEP Medium可以支持方便操作的2D培養，多種品牌的原代肝臟細胞可以存活并維持功能特性4周，以及這款培養基更加人性化，支持隔天換液和周末免換液。

華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司主營細胞治療和精準醫療等產品，2018年與TAKARA繼續合作，授權代理（上海）其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構建試劑。Cellartis公司位于瑞典，擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導技術和ES細胞，ips細胞，分化細胞等干細胞相關產品。

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