BioLife Solutions GMP級CryoStor CS5 Freeze Media細胞DMSO凍存液

產品英文名 BioLife Solutions CryoStor CS5 Freeze Media

產品中文名 BioLife Solutions CryoStor CS5細胞DMSO凍存液

產品介紹  BioLife Solutions CryoStor®是BioLife公司特別優化的細胞凍存液，在極低溫度 (-70ºC to -196ºC)下準備和保存細胞。預混DMSO，為細胞和組織的冷凍、儲存和解凍過程提供安全的保護環境。CryoStor®通過調控凍存過程的分子生物學反應，無血清、蛋白或具有高細胞毒性的試劑，就能增強細胞活力和功能。

產品訂購信息

CryoStor CS5細胞凍存液特點

用于細胞和組織的高級生物保存液

預混DMSO

無血清、無蛋白

USP級高質量成分、cGMP級生產、FDA master文件

滅菌、內毒素和細胞功能檢測

DMSO無血清細胞凍存液適用細胞

多種類型細胞，包括如肝細胞之類的敏感細胞

BioLife Solutions CryoStor CS5 細胞DMSO凍存液cGMP級生產

MATERIALS ARE MANUFACTURED UNDER cGMP

CryoStor® Usage and Cryopreservation Protocol使用與凍存方法

細胞準備

1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2) Centrifuge cells to obtain cell pellet

3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.

加CryoStor凍存液

4) ISOLATION: Add cold (2-8°C) CryoStor®

a. Cell concentrations: 0.5-10 x 106cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor® - no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

儲存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

細胞復蘇解凍

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.Passive thaw is not recommended.

9) Dilute cell/CryoStor® mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.

BioLife Solutions CryoStor CS5/CS10 Freeze Media 細胞凍存液

CS5和CS10主要區別是規格包裝不同。

BioLife Solutions CryoStor Freeze Media GMP級細胞凍存液現貨供應歡迎您咨詢華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司  ：1500 1904 520。紅榮微再-客服:  經銷商專員